CCL2 anticorps

N° du produit ABIN115917

Cet anticorps anti-CCL2 est un anticorps Lapin Polyclonal détectant CCL2 dans WB, EIA et Func. Adapté pour Souris.

Aperçu rapide pour CCL2 anticorps (ABIN115917)

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Reactivité: Souris

Hôte: Lapin

Clonalité: Polyclonal

Conjugué: Cet anticorp CCL2 est non-conjugé

Application: Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)

Détail du produit anti-CCL2 anticorps

Réactivité croisée (Details): Species reactivity (tested):Mouse.

Purification: Affinity Chromatography employing immobilized Murine JE matrix.

Immunogène: Highly pure (>98%) E.coli derived recombinant Mouse JE (MCP-1).

Information d'application

Indications d'application: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine JE(MCP-1)(100 ng/mL), a concentration of 4.0-6.0 μg/mL of this antibody is required. Sandwich ELISA: To detect Murine JE(MCP-1) by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5-2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with Biotinylated Anti-Murine JE(MCP-1)(PP020B1, PP020B2) as adetection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant MurineJE(MCP-1). Western Blot: To detect Murine JE(MCP-1) by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant Murine JE(MCP-1) is 1.5-3.0 ng/lane, under eitherreducing or non-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, pH 7.2 without preservatives.

Agent conservateur: Without preservative

Conseil sur la manipulation: Avoid repeated freezing and thawing. Centrifuge vial prior to opening!

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

Détails sur CCL2

Antigène: CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))

Autre désignation: MCP1 / CCL2

Sujet: This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.Synonyms: C-C motif chemokine 2, HC-11, HC11, MCAF, MCP-1, Monocyte chemoattractant protein 1, Monocyte chemotactic and activating factor, Monocyte chemotactic protein 1, Monocyte secretory protein JE, SCYA2, Small-inducible cytokine A2

ID gène: 9606

UniProt: P13500

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2